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Involvement of MAP kinase activators in angiotensin II‐induced activation of MAP kinases in cultured vascular smooth muscle cells
Author(s) -
Ishida Yoshihiro,
Kawahara Yasuhiro,
Tsuda Terutaka,
Koide Masanobu,
Yokoyama Mitsuhiro
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81142-9
Subject(s) - kinase , mitogen activated protein kinase , vascular smooth muscle , mapk7 , angiotensin ii , microbiology and biotechnology , chemistry , smooth muscle , biochemistry , endocrinology , biology , receptor , extracellular signal regulated kinases
In cultured vascular smooth muscle cells (VSMC) angiotensin II (ang II) induces tyrosine and serine/threonine phosphorylation and activation of two mitogen‐activated protein (MAP) kinases, When extracts of ang II‐stimulated VSMC were fractionated by Mono Q anion‐exchange column chromatography, three peaks of the activities which in vitro activate inactive MAP kinases were detected. These MAP kinase activator activities were not detected in extracts of unstimulated VSMC. In vitro activation of MAP kinases by the MAP kinase activators was accompanied by tyrosine and serine/threonine phosphorylation of MAP kinases. These results suggest that the MAP kinase activators are involved in the ang II‐induced phosphorylation and activation of MAP kinases in VSMC.