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Genomic organization and full‐length cDNA sequence of human collagen X
Author(s) -
Reichenberger Ernst,
Beier Frank,
LuValle Phyllis,
Olsen Bjorn R.,
von der Mark Klaus,
Bertling Wolf M.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81126-7
Subject(s) - untranslated region , polyadenylation , biology , promoter , complementary dna , exon , intron , genetics , microbiology and biotechnology , gene , three prime untranslated region , genomic organization , five prime untranslated region , conserved sequence , messenger rna , peptide sequence , genome , gene expression
We have determined the full‐length cDNA sequence of the human αl(X) collagen gene by sequence analysis of a genomic clone ERG [(1991) Dev. Biol. 148, 562–572], and of cDNA fragments generated from a reverse transcribed as αl(X) mRNA by PCR. We defined the promoter region, the transcription initiation site and the full‐length 5′‐untranslated region. We also report the exon/intron boundaries of the transcript and the complete 3′‐untranslated region as well as a 3′‐flanking sequence containing two additional polyadenylation signals. The promoter region is homologous to chicken and mouse type X promoters within several highly conserved regions. The genomic organization shows high homologies to chicken and mouse.