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cDNA encoding the chicken ortholog of the mouse dilute gene product Sequence comparison reveals a myosin I subfamily with conserved C‐terminal domains
Author(s) -
Sanders Gabriele,
Lichte Beate,
Meyer Helmut E.,
Kilimann Manfred W.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81123-4
Subject(s) - complementary dna , biology , gene , peptide sequence , myosin , gene isoform , gene product , microbiology and biotechnology , conserved sequence , protein primary structure , protein sequencing , subfamily , genetics , biochemistry , gene expression
We report the cDNA‐deduced primary structure of the chicken counterpart of the murine dilute gene product, a member of the myosin I family. Comparison of the chicken and mouse sequences reveals a distinct pattern of domains of high and low sequence conservation. An internal deletion of 25 amino acids probably reflects differential mRNA processing. Compared with other myosin heavy chain molecules, sequence similarity is highest with the MYO2 gene product of Saccharomyces cerevisiae . The MYO2 protein, implicated in vectorial vesicle transport, is homologous to the dilute protein over practically its entire length. In addition, the C‐terminal domain of the dilute protein is highly similar to a putative glutamic acid decarboxylase sequence cloned from mouse brain. Alternatively, this closely related clone might represent an isoform of the dilute protein derived from a second gene, potentially involved in genetic conditions related to dilute.