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Cloning and expression of a novel mouse somatostatin receptor (SSTR2B)
Author(s) -
Vanetti Mirko,
Kouba Monika,
Wang Xiaomin,
Vogt Gudrun,
Höllt Volker
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81122-3
Subject(s) - somatostatin receptor 2 , somatostatin receptor 3 , somatostatin receptor 1 , microbiology and biotechnology , complementary dna , somatostatin receptor , cloning (programming) , peptide sequence , transmembrane domain , messenger rna , biology , transmembrane protein , receptor , chemistry , gene , biochemistry , computer science , programming language
A mouse somatostatin (SS) receptor cDNA was cloned from neuroblastoma x glioma (NG108‐15) cells. The sequence is almost identical to that of the mouse SSTR2 receptor [(1992) Proc. Natl. Acad. Sci. USA 89, 251)] but lacks about 300 nucleotides between transmembrane domain VII and the C‐terminus. This spliced variant of SSTR2 (designated SSTR2B) encodes a protein which is 23 residues shorter than that predicted from the SSTR2 sequence, and differs in 15 amino acids at the C‐terminus. mRNA corresponding to SSTR2B occurs in mouse tissues in higher abundance than that of SSTR2. SSTR2B binds SS peptides with high affinity when expressed in mammalian cells.