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The secondary structure of influenza A M2 transmembrane domain A circular dichroism study
Author(s) -
Duff Kevin C.,
Kelly Sharon M,
Price Nicholas C.,
Bradshaw Jeremy P.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81114-2
Subject(s) - circular dichroism , liposome , transmembrane domain , protein secondary structure , chemistry , transmembrane protein , biophysics , domain (mathematical analysis) , crystallography , membrane , biochemistry , biology , receptor , mathematical analysis , mathematics
Using circular dichroism, this study investigated the secondary structure of the influenza A M2 transmembrane domain. When reconstituted into 1,2‐dioleoyl‐ sn ‐glycero‐3‐phosphocholine liposomes, the M2 transmembrane domain was found to adopt a predominantly α‐helical secondary structure which was unaffected by both temperature and the addition of 1‐aminoadamantane hydrochloride. Reconstitution into 1,2‐dioleoyl‐ sn ‐glycero‐3‐phosphoglycerol liposomes resulted in a marked decrease in helical content.