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Isolation, sequencing and expression in E. coli of the urocanase gene from white clover ( Trifolium repens )
Author(s) -
Koberstaedt Andreas,
Lenz Martin,
Retey János
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81103-s
Subject(s) - trifolium repens , pseudomonas putida , biology , gene , white (mutation) , clone (java method) , genetics , microbiology and biotechnology , escherichia coli , botany
The urocanase gene was detected in a clone obtained from a genomic library of white clover. The entire gene has been sequenced and expressed in the pT7‐7/ E. coli BL 21 (DE 3) system. The deduced sequence of the plant urocanase is 72% homologous with that of the well‐characterized urocanase from Pseudomonas putida . The purification procedure, as well as kinetic and electrophoretic behaviour, of the new enzyme are described.