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Ribosomal localization of the mRNA in the 30S initiation complex as revealed by UV crosslinking
Author(s) -
Brandt Roland,
Gualerzi Claudio O.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81101-q
Subject(s) - 30s , messenger rna , ribosomal rna , ribosomal protein , microbiology and biotechnology , ribosome , transfer rna , nucleic acid , eif4a , translational frameshift , chemistry , translation (biology) , eukaryotic translation , rna , biology , biochemistry , gene
Translation initiation complexes consisting of 30S ribosomal subunits, 32 P‐labelled mRNA (002 mRNA), fMet‐tRNA and the three initiation factors were subjected to UV‐crosslinking to determine the protein and rRNA neighbors of the bound mRNA by immunochemical methods and by nucleic acid hybridization techniques. The mRNA was found to be crosslinked to a specific region of the 16S rRNA spanning from nucleotide 418 to 615 and to ribosomal proteins S1 and S21 (the main targets), S3, S10, S12 and S14; a low level of crosslinking was also detected with S2, S7, S13, S18 and S19.