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Phenylalanyl‐tRNA synthetase from Thermus thermophilus can attach two molecules of phenylalanine to tRNA Phe
Author(s) -
Stepanov Victor G.,
Moor Nina A.,
Ankilova Valenti.,
Lavrik Ol'ga I.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81099-8
Subject(s) - thermus thermophilus , transfer rna , thermophile , phenylalanine , thermus aquaticus , thermus , enzyme , biochemistry , chemistry , stereochemistry , escherichia coli , amino acid , rna , gene
Phenylalanyl‐tRNA synthetase from the extreme thermophilic bacterium Thermus thermophilus can incorporate more than one molecule of phenylalanine into the tRNA Phe . It is shown that the ‘hyperaminoncylated’ tRNAP Phe is the bis‐2′,3′‐ O ‐phenylalanyl‐tRNA Phe , and its formation is typical for the thermophilic enzyme but does not occur for E. coli phenylalanyl‐tRNA synthetase under the same conditions.