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The role of the C‐terminal region of rat brain natriuretic peptide in receptor selectivity
Author(s) -
Shimekake Yoshiyuki,
Kawabata Tomoji,
Nakamura Masuhisa,
Nagata Kiyoshi
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81091-y
Subject(s) - receptor , npr2 , atrial natriuretic peptide , peptide , npr1 , medicine , chemistry , endocrinology , brain natriuretic peptide , natriuretic peptide , terminal (telecommunication) , microbiology and biotechnology , biology , biochemistry , heart failure , telecommunications , computer science
Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) have different C‐terminal tail structures compared with the rather conservative ring structures which consist of 17 amino acid residues. To examine the different effects of the tail structures of ANP and BNP on their interaction with receptors, we synthesized several peptide analogs and measured their biological actions in three different assay systems. Deletion of the C‐terminal tail from rat BNP did not effect the vasorelaxation activity against rat aorta, but it promoted cGMP production in cultured rat aortic smooth muscle cells (RASMC). Deletion of the C‐terminal tail from rat ANP diminished both vasorelaxant and cGMP producing activities. In a binding competition assay with RASMC and [ 125 I]rat ANP‐(1–28), the competition activities of both ANP and BNP were greatly reduced by C‐terminal deletion. In addition, we obtained agonists with novel receptor selectivity.