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Antibody to the inositol trisphosphate receptor blocks thimerosalenhanced Ca 2+ ‐induced Ca 2+ release and Ca 2+ oscillations in hamster eggs
Author(s) -
Miyazaki Shun-ichi,
Shirakawa Hideki,
Nakada Ken,
Honda Yoshiko,
Yuzaki Michisuke,
Nakade Shinji,
Mikoshiba Katsuhiko
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81090-9
Subject(s) - thimerosal , inositol , chemistry , inositol trisphosphate , inositol trisphosphate receptor , inositol phosphate , biophysics , hamster , receptor , cytosol , intracellular , biochemistry , microbiology and biotechnology , biology , enzyme , medicine , dermatology
The sulfhydryl reagent thimerosal enhanced the sensitivity of hamster eggs to injected inositol 1,4,5‐trisphosphate (InsP 3 ) or Ca 2+ to generate regenerative Ca 2+ release from intracellular pools. A monoclonal antibody (mAb) to the InsP 3 receptor blocked both the InsP 3 ‐induced Ca 2+ release (IICR) and Ca 2+ ‐induced Ca 2+ release (CICR). The mAb also blocked Ca 2+ oscillations induced by thimerosal. The results indicate that thimerosal enhances IICR sensitized by cytosolic Ca 2+ , but not CICR from InsP 3 ‐insensitive pools, and causes repetitive Ca 2+ releases from InsP 3 ‐sensitive pools.