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Lipid interaction of Tetanus neurotoxin A calorimetric and fluorescence spectroscopy study
Author(s) -
Calappi Emanuela,
Masserini Massimo,
Schiavo Giampietro,
Montecucco Cesare,
Tettamanti Guido
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81074-v
Subject(s) - fluorescence spectroscopy , chemistry , fluorescence , neurotoxin , biophysics , tetanus , spectroscopy , biochemistry , biology , virology , physics , quantum mechanics , vaccination
The interaction of Tetunus toxin with phospholipid vesicles containing gangliosides (GD1a, GD1b or GT1b) or phosphatidic acid has been investigated at neutral or acidic pH. Change in the thermotropic properties of the vesicles occurred only after addition of the toxin at acidic pH, and led to surface binding or membrane insertion of the protein, dependent on the physical state of the membrane. Most remarkably, toxin addition at acidic pH to dipalmitoyl‐phosphatidylcholine vesicles containing GT1b ganglioside, caused formation of ganglioside microdomains on the vesicle surface.