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Single channel recording in the chloroplast envelope
Author(s) -
Pottosin Igor I.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81057-s
Subject(s) - conductance , chloroplast membrane , ion channel , patch clamp , biophysics , envelope (radar) , chemistry , channel (broadcasting) , chloroplast , membrane potential , voltage clamp , membrane , ion , electrophysiology , biology , physics , biochemistry , telecommunications , computer science , neuroscience , condensed matter physics , receptor , thylakoid , radar , gene , organic chemistry
The patch‐clamp technique was applied to study ion channels in the intact chloroplast envelope. Three channel types were characterized: two cation‐selective, with a conductance (in 100 mM KCl) of 517 and 1016 pS, respectively, and one anion‐selective with a conductance of 159 pS. All three channels showed voltage‐dependent closures at both positive and negative membrane potentials.