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The apical sorting signal on human aminopeptidase N is not located in the stalk but in the catalytic head group
Author(s) -
Vogel Lotte Katrine,
Norén Ove,
Sjöström Hans
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81039-o
Subject(s) - aminopeptidase , stalk , signal peptide , biology , biochemistry , microbiology and biotechnology , chemistry , peptide sequence , amino acid , leucine , gene , horticulture
Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin‐Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk‐minus aminopeptidase N, consisting of the hemagglutinin signal peptide and the catalytic head group of human aminopeptidase N, was expressed in MDCK cells. Anchor/stalk‐minus aminopeptidase N was secreted mainly to the apical side. The catalytic head group of human aminopeptidase N thus carries an apical sorting signal.