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Carbon‐by‐carbon discrimination of 13 C incorporation into liver fatty acids
Author(s) -
Cunnane S.C.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81016-f
Subject(s) - fatty acid , chemistry , carbon 14 , carbon fibers , metabolism , fatty acid synthesis , biochemistry , fatty acid metabolism , polyunsaturated fatty acid , carbon 13 , organic chemistry , physics , materials science , quantum mechanics , composite number , composite material
Quantitative carbon‐by‐carbon analysis would be useful in determining the origin and fate of carbons involved in fatty acid metabolism. Incorporation of 13 C from 2‐[ 13 C]acetate into specific carbons of liver fatty acids was lowest at the n—2 carbon of saturates and monoenes but was 47% greater at acyl Cl than at C2, suggesting substantial redistribution of the 13 C from C2 to Cl of acetyl CoA or malonyl CoA prior to 13 C incorporation into fatty acids during de novo synthesis or during elongation. Thus, 13 C derived from exogenous acetate can be quantitatively measured and is differentially incorporated into individual carbons depending on position in the fatty acid molecule.