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Molecular cloning of a novel ras‐like protein from chicken
Author(s) -
Trueb Judith,
Trueb Beat
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80995-s
Subject(s) - complementary dna , biochemistry , biology , amino acid , peptide sequence , ras superfamily , gtp' , cloning (programming) , cdna library , microbiology and biotechnology , gene , enzyme , programming language , computer science
We have isolated a cDNA clone from a chicken DNA expression library which codes for a ras‐like polypeptide of 216 amino acid residues. This polypeptide is closely related to the human protein TC4 and to the yeast protein Spil, two novel proteins that may be involved in the coordination of the cell cycle. In the amino‐terminal region, the three polypeptides possess a P‐loop motif characteristic of GTP‐binding proteins. At the carboxy‐terminal end, however, they lack the typical CAAX‐box which is usually responsible for membrane anchorage of ras‐like proteins. It is therefore likely that the three polypeptides define a new subclass of GTP‐binding proteins within the ras‐like superfamily.