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Evidence for the tetraplex structure of the d(GT) n repetitive sequences in solution
Author(s) -
Borisova Olga F.,
Shchyolkina Anna K.,
Timofeev Edward N.,
Florentiev Vladimir L.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80985-p
Subject(s) - ethidium bromide , circular dichroism , oligonucleotide , chemistry , intercalation (chemistry) , crystallography , bromide , molecule , fluorescence , stereochemistry , dna , biochemistry , inorganic chemistry , organic chemistry , physics , quantum mechanics
The ability of oligonucleotides 3′‐d(GT) 5 pO(CH 2 ) 6 Opd(GT) 5 ‐5′(anti[d(GT)]) and 3′‐d(GT) 5 pO(CH 2 ) 6 Opd(GT) 5 ‐3′(par[d(GT)]) to form tertiary structures has been studied. Circular dichroism (CD) as well as the fluorescence of the ethidium bromide (E1Br) complexes with oligonucleotides and hydrodynamic volume measurements in solutions containing 0.01 M phosphate buffer, pH 7 and NACl in concentrations from 0.1 M to 1 M, have been used. The data obtained in the temperature interval from 30°C to 10°C are in good agreement with the structure suggested earlier [1] where the par[d(GT)] and anti[d(GT)] form structures with four parallel strands in which layers of four G‐residues alternate with unpaired bulged‐out T‐residues. Ethidium bromide interacts with the structure in a cooperative manner. Two ethidium bromide molecules intercalate between two layers of four G‐residues.