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On the functional role of the Tyr‐639 residue of bacteriophage T7 RNA polymerase
Author(s) -
Rechinsky V.O.,
Kostyuk D.A.,
Tunitskaya V.L.,
Kochetkov S.N.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80983-n
Subject(s) - rna polymerase , polymerase , rna dependent rna polymerase , rna , nucleotide , bacteriophage , enzyme , residue (chemistry) , t7 rna polymerase , biochemistry , gtp' , chemistry , biology , microbiology and biotechnology , escherichia coli , gene
Substitution or Asp for a Tyr residue normally present at position 639 of the bacteriophage T7 RNA polymerase leads to a drastic drop in the enzymatic activity. This mutation does not affect the enzyme‐promoter interaction but decreases the ability of the RNA polymerase to discriminate between GTP and ATP molecules, resulting in a decrease in the rate of the incorporation of the nucleotide into the RNA chain.