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Photogeneration of NADH under coupled action of CdS semiconductor and hydrogenase from Alcaligenes eutrophus without exogenous mediators
Author(s) -
Shumilin I.A.,
Nikandrov V.V.,
Popov V.O.,
Krasnovsky A.A.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80982-m
Subject(s) - formate , nad+ kinase , hydrogenase , chemistry , photochemistry , sodium formate , electron transport chain , cofactor , formate dehydrogenase , electron transfer , dimer , enzyme , inorganic chemistry , biochemistry , catalysis , organic chemistry
Photoreduction of NAD has been accomplished by a system consisting of the NAD‐dependent hydrogenase from Alcaligenes eutrophus immobilized on CdS particles with formate as artificial electron donor. Enzymatically active NADH is formed under illumination of this system by visible light. Accumulation of the coenzyme dimer (NAD) 2 was not detected. NAD photoreduction is supposed to proceed via the direct electron transfer from the semiconductor to the enzyme electron transport chain. However, NADH formation as a result of hydrogenase interaction with anion‐radicals (CO .− 2 )‐formed in the course of formate photooxidation cannot at present be excluded.