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cDNA sequence analysis of an antibiotic dodecapeptide from neutrophils
Author(s) -
Storici Paola,
Del Sal Giannino,
Schneider Claudio,
Zanetti Margherita
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80971-i
Subject(s) - complementary dna , sequence (biology) , antibiotics , sequence analysis , biology , computational biology , genetics , microbiology and biotechnology , dna , gene
The full‐length cDNA of a neutrophil antibiotic dodecapeptide has been cloned by reverse transcription/PCR from bovine bone marrow RNA. This peptide was originally isolated from bovine neutrophils, and shown to exert a potent antimicrobial activity in vitro on both Escherichia coli and Staphylococcus aureus . The cDNA codes for a polypeptide of 155 amino acid residues with a predicted mass of 17,629 Da and a pl of 8.03. The deduced sequence comprises a putative signal peptide of 29 amino acids, a 114 residue pro‐region, and a carboxy‐terminal dodecapeptide corresponding to the mature antibiotic. The pro‐sequence displays extensive identity to corresponding regions of other structurally unrelated antibiotic peptides of bovine neutrophils recently cloned.