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Gradient expression of Cdx along the rat intestine throughout postnatal development
Author(s) -
Freund Jean-Noël,
Boukamel Rita,
Benazzouz Aziz
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80965-j
Subject(s) - biology , northern blot , messenger rna , microbiology and biotechnology , genomic dna , gene , small intestine , southern blot , gene expression , western blot , genome , andrology , genetics , endocrinology , medicine
Rat genomic DNA was isolated by homology with Cdxl , a murine homeogene selectively expressed in intestinal cells of endodermal origin. Southern blot analysis indicated that the rat genome contains a single or a small number of closely related Cdx gene(s). A major 1.7 kb Cdx mRNA was detected in neonate, suckling and adult rats whereas a 6.5 kb mRNA was restricted to sucklings and adults. Both transcripts showed decreasing concentration from the colon towards the proximal part of the small intestine. No obvious correlation could be established with the patterns of expression of transcripts corresponding to markers of cell proliferation and cell differentiation during postnatal development.