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Assignment of the redox potentials to the four haems in Desulfovibrio vulgaris cytochrome c 3 by 2D‐NMR
Author(s) -
Salgueiro Carlos A.,
Turner David L.,
Santos Helena,
LeGall Jean,
Xavier António V.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80963-h
Subject(s) - desulfovibrio vulgaris , desulfovibrio , redox , cytochrome , chemistry , proton nmr , cytochrome c , crystallography , stereochemistry , bacteria , inorganic chemistry , biochemistry , biology , organic chemistry , mitochondrion , enzyme , genetics , sulfate
Using 2D‐NMR the four haems of Desulfovibrio vulgaris (Hildenborough) cytochromes, within the X‐ray structure were fully cross‐assigned according to their redox potential. The strategy used was based on a complete network of chemical exchange connectivities between the NMR signals obtained for all oxidation levels to the corresponding ones in the fully reduced spectrum [1992, Eur. J. Biochem., in press]. This unequivocal cross‐assignment disagrees within earlier results obtained for the similar protein from Desulfovibrio vulgaris (Miyazaki F.) [1991, FEBS Lett. 285, 149–151]