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Noradrenaline release from permeabilized synaptosomes is inhibited by the light chain of tetanus toxin
Author(s) -
Stecher Brigitte,
Hens Jacques J.H.,
Weller Ulrich,
Gratzl Manfred,
Gispen Willem-Hendrik,
De Graan Pierre N.E.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80933-8
Subject(s) - toxin , streptolysin , tetanus , synaptosome , immunoglobulin light chain , chemistry , clostridium tetani , biochemistry , biophysics , in vitro , biology , antibody , immunology , bacterial protein , vaccination , gene
Noradrenaline release from rat brain cortical synaptosomes permeabilized with streptolysin O can be triggered by μM concentrations of free Ca 2+ . This process was inhibited within minutes by tetanus toxin and its isolated light chain, but not by its heavy chain. The data demonstrate that the effect of tetanus toxin on NA release from purified synaptosomes is caused by the intraterminal action of its light chain.