Premium
Characterisation of the expression and post‐translational processing of the preprotachykinin‐I gene and the regulated release of tachykinins by the RINm5F cell‐line
Author(s) -
McGregor Gerard P.,
Hartel Robert,
Fehmann Hans-Christoph,
Lankat-Buttgereit Brigitte,
Göke Burkhard,
Göke Rüdiger,
Voigt Karlheinz
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80932-7
Subject(s) - gene , gene expression , microbiology and biotechnology , cell culture , chemistry , line (geometry) , biology , biochemistry , genetics , mathematics , geometry
Normal transcription and postranslational processing of the preprotachykinin (PPT)‐I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell‐line, RINm5F, has been demonstrated, using radioimmunoassays (RlAs), reversed‐phase (rp)HPLC and Northern blot analysis. This is the first stable cell‐line found to express the PPT‐I gene and provides an opportunity for investigating PPT‐I gene expression and tachykinin biosynthesis. RIN5mF cells are a model for the pancreatic beta‐cell, which is not known to exhibit PPT‐I gene expression which may, therefore, be a feature of the transformed state of these cells. These data may imply that the tachykinins are important in pancreatic islet embryogenesis.