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Expression of functional Thermoplasma acidophilum proteasomes in Escherichia coli
Author(s) -
Zwickl Peter,
Lottspeich Friedrich,
Baumeister Wolfgang
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80925-7
Subject(s) - thermoplasma acidophilum , escherichia coli , proteasome , biology , recombinant dna , biochemistry , gene , enzyme
The two genes encoding the constituent subunits of the Thermoplasma acidophilum proteasome were expressed in Escherichia coli yielding fully assembled molecules as shown by electron microscopy. The recombinant proteasomes were purified to homogeneity and ware shown to have proteolytic activity indistinguishable from proteasomes isolated from T. acidophilum .