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Peptide display on filamentous phage capsids An new powerful tool to study protein—ligand interaction
Author(s) -
Cesareni Gianni
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80903-t
Subject(s) - phage display , capsid , ligand (biochemistry) , chemistry , peptide , filamentous bacteriophage , bacteriophage , computational biology , biophysics , biochemistry , biology , escherichia coli , receptor , gene
Peptides can be displayed on the surface of filamentous bacteriophages by fusion to phage coat proteins. It was recently shown that vast (10 8 ) collections of phages, each exposing a variant of the original peptide, can be constructed and utilized as a general source of peptide ligands. By panning these libraries on a target molecule linked to a solid support it is possible to select, out of the hundreds of millions of clones, those few phages that display a peptide that binds the target molecule. Searching these libraries is a powerful tool to be applied in many areas of fundamental and applied biology.