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Calcium administration stimulates the expression of calcium‐binding protein regucalcin mRNA in rat liver
Author(s) -
Shimokawa Noriaki,
Yamaguchi Masayoshi
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80884-j
Subject(s) - messenger rna , calcium binding protein , endocrinology , calcium , medicine , northern blot , western blot , gene expression , biology , kidney , microbiology and biotechnology , chemistry , gene , biochemistry
The distribution and expression of mRNA encoding the Ca 2+ ‐binding protein regucalcin in rats were investigated by Northern blot analyses. Liver regucalcin cDNA (0.6 kb) was used as a probe. The analyses of total RNAs extracted from various tissues of rat indicated that regucalcin mRNA was mainly present in liver but only slightly in kidney with a size of 1.8 kb. The expression level decreased with increasing age (3, 10 and 25 weeks). A single intraperitoneal administration of calcium chloride (15 mg Ca/100 g body weight) induced a remarkable increase in regucalcin mRNA in liver; the level was about 200% of control at 30 min after the administration. Subsequently, the expression level began to decrease with time and was about 40% of control level at 120 min after the administration. The increase in regucalcin mRNA levels at 30 min after calcium administration was dose‐dependent. These observations show that the expression of regucalcin mRNA is specific in liver of various tissues, and that it is regulated by Ca 2+ administration. Regucalcin may have a role as regulatory protein for calcium homeostasis in liver cells.