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Cloning and expression of a glycine transporter from mouse brain
Author(s) -
Liu Qing-Rong,
Nelson Hannah,
Mandiyan Sreekala,
López-Corcuera Beatriz,
Nelson Nathan
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80875-h
Subject(s) - cloning (programming) , expression cloning , transporter , glycine , chemistry , biology , biochemistry , gene , amino acid , complementary dna , computer science , programming language
We have isolated a cDNA clone from a mouse brain library encoding the glycine transporter (GLYT). Xenopus oocytes injected with a synthetic mRNA accumulated [ 3 H]glycine to levels of up to 80‐fold above control values. The uptake was specific for glycine and dependent on the presence of Na + and Cl − in the medium. The cDNA sequence predicts a highly hydrophobic protein of 633 amino acids with 12 potential transmembrane helices. The predicted amino acid sequence has 40–45% identity to the GABA, noradrenaline, serotonin and dopamine transporters. This implies that all of these neurotransmitter, transporters may have evolved from a common ancestral gene that diverged into the GABA, glycine and catecholamine subfamilies at nearly the same time.