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The effect of the UL42 protein on the DNA polymerase activity of the catalytic subunit of the DNA polymerase encoded by herpes simplex virus type 1
Author(s) -
Hart Graham J.,
Boehme Richard E.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80872-e
Subject(s) - dna polymerase , polymerase , protein subunit , dna polymerase delta , microbiology and biotechnology , dna polymerase ii , single stranded binding protein , dna clamp , chemistry , dna , biology , biochemistry , dna binding protein , gene , polymerase chain reaction , reverse transcriptase , transcription factor
The effect that the UL42 protein of herpes simplex virus type 1 has on the DNA polymerase activity of the DNA polymerase catalytic subunit (Pol) of the same virus has been investigated. The observed effects are critically dependent on the salt used and its concentration, such that the UL42 protein may inhibit, have little or no effect on, or activate the Pol activity, depending on the conditions used. The observed effects are due to the values for K m app for activated DNA and V max app for Pol and the Pol‐UL42 protein complex differently varying with salt concentration.