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Heat‐labile enterotoxin crystal forms with variable A/B 5 orientation Analysis of conformational flexibility
Author(s) -
Sixma Titia K.,
Aguirre Angel,
van Scheltinga Anke C.Terwisscha,
Wartna Ellen S.,
Kalk Kor H.,
Hol Wim G.J.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80869-i
Subject(s) - pentamer , chemistry , crystallography , protein subunit , crystal (programming language) , flexibility (engineering) , crystal structure , stereochemistry , biochemistry , mathematics , statistics , computer science , gene , programming language
A new native crystal form of heat‐labile enterotoxin (LT) has two AB 5 complexes in the asymmetric unit with different orientations of the A subunit with respect to the B pentamer. Comparison with other crystal forms of LT shows that there is considerable conformational freedom for orientating the A subunit with respect to the B pentamer. The rotations of A in different crystal forms do not follow one specific axis, but most of them share a hinge point, close to the main interaction area between A and B 5 . Analysis of the two high‐resolution structures available shows that these rotations cause very little change in the actual interactions between A and B 5 .