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Immunocharacterization of δ‐ and ζ‐isoenzymes of protein kinase C in rat renal mesangial cells
Author(s) -
Huwiler Andrea,
Fabbro Doriano,
Stabel Silvia,
Pfeilschifter Josef
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80858-e
Subject(s) - isozyme , chemistry , protein kinase c , protein kinase a , kinase , medicine , microbiology and biotechnology , biochemistry , biology , enzyme
The isoforms of protein kinase C (PKC) present in rat mesangial cells were identified by immunoblot analysis with antibody raised against isotype‐specific peptides. In addition to the previously observed α‐ and ε‐subspecies, mesangial cells also express the δ‐ and ζ‐isoenzymes of PKC. On exposure to phorbol 12,13‐dibutyrate (PDB) a complete depletion of PKC‐δ is observed within 8 h. Removal of PDB results in a recovery of PKC‐δ. In contrast, PKC‐ζ is unaffected by addition or removal of PDB.