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Sphingolipid transport from the trans‐Golgi network to the apical surface in permeabilized MDCK cells
Author(s) -
Kobayashi Toshihide,
Pimplikar Sanjay W.,
Parton Robert G.,
Bhakdi Sucharit,
Simons Kai
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80851-7
Subject(s) - sphingolipid , sphingomyelin , golgi apparatus , vesicle , microbiology and biotechnology , apical membrane , ceramide , biochemistry , biology , cytosol , biophysics , membrane , endoplasmic reticulum , enzyme , apoptosis
We have measured the transport of de novo synthesized fluorescent analogs of sphingomyelin and glucosylceramide from the trans‐Golgi network (TGN) to the apical membrane in basolaterally permeabilized Madin‐Darby canine kidney (MDCK) cells. Sphingolipid transport was temperature, ATP and cytosol dependent. Introduction of bovine serum albumin (BSA), which binds fluorescent sphingolipid monomer, into the permeabilized cells, did not affect lipid transport to the apical membrane. Both fluorescent sphingomyelin and glucosylceramide analogs were localized to the lumenal bilayer leaflet of isolated TGN‐derived vesicles. These results strongly suggest that both sphingolipids are transported from the TGN to the apical membrane via vesicular traffic.