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The nucleotide sequence of the S RNA of Impatiens necrotic spot virus, a novel tospovirus
Author(s) -
de Haan Peter,
de Avila Antonio C.,
Kormelink Richard,
Westerbroek Annemarie,
Gielen Jan J.L.,
Peters Dick,
Goldbach Rob
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80830-a
Subject(s) - tospovirus , biology , virology , bunyaviridae , nucleic acid sequence , rna , impatiens , plant virus , virus , genetics , gene , tomato spotted wilt virus , botany , cultivar
Impatiens necrotic spot virus (INSV) shares a number of properties with tomato spotted wilt virus (TSWV), the type species of the genus tospovirus within the family Bunyaviridae. INSV, however, differs from TSWV in plant host range and serology. In order to define the genomic structure and the taxonomic status of this TSWV‐Iike virus, the nucleotide sequence of its genomic S RNA segment has been determined. The molecular data obtained demonstrate that, like TSWV, INSV has an ambisense S RNA molecule, encoding a non‐structural protein in viral sense and the nucleocapsid protein in viral complementary sense. The level of nucleotide sequence homology between their S RNAs, as well as the divergence in amino acid sequence homology of their gene products, confirm previous conclusions from serological studies that INSV and TSWV represent distinct virus species within the newly created genus, tospovirus.