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Molecular cloning and functional expression of the second mouse nm 23/NDP kinase gene, nm 23‐M2
Author(s) -
Urano Takeshi,
Takamiya Kogo,
Furukawa Koichi,
Shiku Hiroshi
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80807-s
Subject(s) - microbiology and biotechnology , complementary dna , open reading frame , fusion protein , gene , cdna library , gene expression , messenger rna , kinase , coding region , molecular cloning , gene product , biology , cloning (programming) , fusion gene , chemistry , peptide sequence , biochemistry , recombinant dna , computer science , programming language
A new murine cDNA of nm 23/NDP kinase was isolated. A RT‐PCR product was obtained from the normal mouse liver mRNA with primers designed for the human nm 23‐H2 gene. The product was used as a probe to screen a cDNA library from the murine melanoma cell line. B16, and two clones containing the entire open reading frame were obtained. It was predicted that the DNA sequence encoded 152 amino acids which was 98% identical to the nm 23‐H2 protein. The entire nm 23‐M1 and ‐M2 gene‐coding regions were translated as fusion proteins with a glutathione S ‐transferase. These fusion proteins displayed NDP kinase activities.