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Carbohydrates in mammalian tryptophanyl‐tRNA synthetase
Author(s) -
Kovaleva Galina K.,
Zheltova Anna O.,
Nikitushkina Tatyana V.,
Egorov Tsezi A.,
Musoljamov Aleksander Ch.,
Kisselev Lev L.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80802-n
Subject(s) - biochemistry , fucose , mannose , enzyme , aminoacyl trna synthetase , chemistry , galactose , monosaccharide , transfer rna , phosphoprotein , glycoprotein , aminoacylation , molecular mass , biology , rna , gene
Homogeneous preparations of bovine tryptophanyl‐tRNA synthetase (EC 6.1.1.2) contain monosaccharides (mannose, fucose, galactose, N ‐acetylglucosamine) as revealed by liquid chromatography. Their content comprises 2.5–3.0% (w/w) of the enzyme composed of two subunits (60 kDa × 2). The same set of sugars was detected in clastase and CNBr‐generated fragments (with molecular masses of approx. 40 kDa and 30 kDa, respectively). It is concluded that bovine tryptophanyl‐tRNA synthetase, in addition to being a metallo‐ and phosphoprotein, is also a glycoprotein.