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A calcineurin‐like phosphatase is required for catch contraction
Author(s) -
Castellani Loriana,
Cohen Carolyn
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80798-l
Subject(s) - calcineurin , phosphatase , calmodulin , contraction (grammar) , alkaline phosphatase , chemistry , microbiology and biotechnology , biology , biochemistry , endocrinology , medicine , enzyme , transplantation
The ability of certain molluscan smooth muscles to maintain a prolonged state of contraction, termed ‘catch’, has been correlated with the activity of a calcineurin‐like Ca 2+ ‐regulated phosphatase. The release of this phosphatase through extensive treatment of fibers with detergent, as shown by Western blots and a calmodulin‐binding overlay assay, results in the loss of catch tension maintenance. This effect is reversed by perfusion of the fiber with brain calcineurin. These findings suggest that the activity of the calcineurin‐like phosphatase, switched on during the onset of active contraction, plays a critical role in the maintenance of catch.