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Cloning and sequence analysis of a cDNA clone from Arabidopsis thaliana homologous to a proteasome α subunit from Drosophila
Author(s) -
Genschik Pascal,
Philipps Gabriel,
Gigot Claude,
Fleck Jacqueline
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80796-j
Subject(s) - arabidopsis thaliana , cloning (programming) , complementary dna , proteasome , protein subunit , biology , clone (java method) , homologous chromosome , drosophila (subgenus) , genetics , arabidopsis , sequence (biology) , microbiology and biotechnology , computational biology , gene , mutant , computer science , programming language
A cDNA clone isolated from an Arabidopsis thaliana cell suspension culture library showed considerable similarities to the proteasome 28 kDa α subunit of Drosophila [(1990) Gene 90, 235–241]. The 250 amino acid‐long protein encoded by Arabidopsis TAS‐g64 clone has important homologies in its primary structure and in the predicted secondary structure with the PROS‐28.1 clone from Drosophila . The only divergence observed between the two sequences is for the 20 C‐terminal amino acids. This subunit might share important functions in both kingdoms, as revealed by the important conservation between plants and animals. In plant cells it is encoded by a single‐copy gene and probably regulated by stress and/or division.