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Direct transfer of molybdopterin cofactor to aponitrate reductase from a carrier protein in Chlamydomonas reinhardtii
Author(s) -
Aguilar Miguel,
Kalakoutskii Kyrill,
Cárdenas Jacobo,
Fernández Emilio
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80758-9
Subject(s) - chlamydomonas reinhardtii , cofactor , chemistry , microbiology and biotechnology , biochemistry , biology , enzyme , gene , mutant
A Chlamydomonas reinhardtii molybdenum cofactor (MoCo)‐carrier protein (CP), capable of reconstituting nitrate reductase activity with apoprotein from the Neurospora crassa mutant nit ‐1, was subjected to experiments of diffusion through a dialysis membrane and gel filtration. CP bonded firmly MoCo and did not release it efficiently unless aponitrate reductase was present in the incubation mixture, Stability of MoCo bound to CP against air and heat was very similar to that of free‐MoCo released from milk xanthine oxidase. Our data strongly suggest that MoCo is directly transferred from CP to aponitrate reductase to form an active enzyme.