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Protein kinase Cζ and n in murine epidermis TPA induces down‐regulation of PKC n but not PKCζ
Author(s) -
Gschwendt Michael,
Leibersperger Hanno,
Kittstein Walter,
Marks Friedrich
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80756-7
Subject(s) - protein kinase c , bryostatin 1 , staurosporine , microbiology and biotechnology , cytosol , chemistry , pkc alpha , biology , biochemistry , kinase , enzyme
Murine epidermis contains PKCζ and n as evidenced by the application or specific antisera. PKCζ predominates in the cytosol and PKC n in the particulate fraction. PKCζ is shown to be present also in other murine tissues, with large amounts found in lung. Whereas epidermal PKC n is completely down‐regulated by treatment or mouse skin with TPA or bryostatin 1 for 18 h. PKCζ is neither translocated by treatment with TPA for 20 min, nor down‐regulated by treatment with TPA or bryostatin 1 for 18 h. PKCζ is activated by phosphatidyl serine alone and does neither respond to Ca 2+ nor to TPA. It is inhibited by staurosporine with an IC 50 of 16 nM, which is within the same range or other PKC isoenzymes. The sensitivity of PKCζ towards the staurosporine derivative K252a is similar to that or PKCα,β,γ but much higher than that of PKCδ and ε.