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Interaction of plasma gelsolin with tropomyosin
Author(s) -
Koepf Edward K.,
Burtnick Leslie D.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80738-3
Subject(s) - gelsolin , tropomyosin , actin , chemistry , microbiology and biotechnology , computational biology , biochemistry , biology
Horse plasma gelsolin labelled with benzophenone‐4‐isothiocyanate can be photochemically cross‐linked to rabbit cardiac tropomyosin. The cross‐linking proceeds with greater efficiency in calcium‐containing buffers. Further evidence for interaction between these proteins is provided by retention of fluorescently labelled gelsolin on tropomyosin‐agarose affinity columns and by the ability of tropomyosin to cause an increase in the fluorescence intensity of gelsolin labelled with fluorescein‐5‐isothiocyanate. Both of these effects require the presence of calcium ions.