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Antifungal activity of chitin‐binding PR‐4 type proteins from barley grain and stressed leaf
Author(s) -
Hejgaard Jørn,
Jacobsen Susanne,
Bjørn Søren E.,
Kragh Karsten M.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80720-2
Subject(s) - pathogenesis related protein , chitin , chitinase , biology , biochemistry , trichoderma harzianum , hordeum vulgare , in vitro , fusarium , microbiology and biotechnology , botany , poaceae , enzyme , solanaceae , gene , biological pest control , chitosan
Antifungal activity in vitro has been associated with barley leaf and grain proteins which are homologous with pathogenesis related proteins of type 4 (PR‐4) from tobacco and tomato and with C terminal domains of potato win and Hevea hevein precursor proteins. One protein (pI ∼9.3, M r ∼13.7 kDa) from barley grain and two very similar proteins from leaves infected with Erysiphe graminis were isolated by chitin affinity chromatography, but none of the proteins showed chitinase activity in vitro. The leaf proteins were increased several fold in response to either Erysiphe infection or NiCl 2 infiltration and accumulated extracellularly. The three barley proteins were found to inhibit growth of Trichoderma harzianum in microtiter plate assays using ∼‐10 μg/ml concentrations and in lower concentrations in a synergistic way when mixed either with barley chitinase C (a PR‐3 type protein) or with barley protein R (a PR‐5 type protein). Structurally similar proteins were detected in wheat, rye and oats grain extracts.