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Crosslinking of nuclear proteins to DNA by cis ‐diammminedichloroplatinum in intact cells Involvement of nuclear matrix proteins
Author(s) -
Ferraro Anna,
Grandi Paolo,
Eufemi Margherita,
Altieri Fabio,
Turano Carlo
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80718-v
Subject(s) - nuclear matrix , nuclear protein , dna , nuclear dna , matrix (chemical analysis) , gel electrophoresis , dna binding protein , chemistry , cell nucleus , microbiology and biotechnology , cell , biology , biochemistry , transcription factor , gene , chromatin , mitochondrial dna , chromatography
In order to detect the nuclear matrix proteins involved in DNA binding, avoiding possible artifacts derived from the disruption of nuclei, proteins were crosslinked to DNA by the action of cis ‐diamminedichloroplatinum on intact chicken liver cells and analyzed by two‐dimensional gel electrophoresis. At least eleven species of crosslinked proteins were found to derive from the nuclear matrix prepared from the same cell type, and five of these were found also among the proteins crosslinked to DNA in intact liver cells from ox and pig. This subset of common proteins, conserved in different animal species, is likely to have a fundamental role for the anchorage of DNA to the nuclear matrix.