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Abolishment of bradykinin‐induced calcium oscillations in ras ‐transformed fibroblasts by the expression of 80 kDa diacylglycerol kinase
Author(s) -
Fu Tao,
Sugimoto Yoshikazu,
Okano Yukio,
Kanoh Hideo,
Nozawa Yoshinori
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80700-q
Subject(s) - diacylglycerol kinase , protein kinase c , transfection , bradykinin , kinase , microbiology and biotechnology , pi , biology , chemistry , cell culture , biochemistry , receptor , genetics
Our previous study showed bradykinin‐induced periodic Ca 2+ changes (Ca 2+ oscillations) in v ‐Ki‐ ras ‐transformed NIH/3T3 (DT) cells in which protein kinase C (PKC) activity is partially down‐regulated by a sustained high level of 1,2‐diacylglycerol (DAG) [FEBS Lett. (1991) 281. 263–266].In the present study, DAG kinase with 80 kDa mass (80K DGK) has been successfully transfected in DT cells, which exhibited enhanced cellular DAG kinase activities, decreased cellular DAG contents, and increased PKC activities compared to the control vector‐transfected cells. Furthermore, these DGK‐transfectants showed strong inhibition in bradykinin‐induced Ca 2+ oscillations. The results suggest that the sustained DAG increase down‐regulates the PKC activity, thereby leading to the induction of Ca 2+ oscillations in DT cells.