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Cell density‐dependent expression of heparin‐binding growth‐associated molecule (HB‐GAM, p18) and its down‐regulation by fibroblast growth factors
Author(s) -
Merenmies Jussi
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80699-h
Subject(s) - cell growth , microbiology and biotechnology , cell culture , biology , cell division , 3t3 cells , messenger rna , cell cycle , fibroblast , cell , chemistry , gene , genetics , transfection
Heparin‐binding growth‐associated molecule (HB‐GAM) is a developmentally regulated protein that is intensely expressed during the rapid postnatal growth phase of rat brain. The expression of HB‐GAM studied in 12 cell lines was restricted to C6 rat glioma cell line and BALB/c 3T3 cells. In BALB/c 3T3 cells the expression of HB‐GAM enhanced in confluent and quiescent cell cultures. When the confluent cultures were treated with bFGF the expression of HB‐GAM mRNA was strongly reduced and the protein disappeared rapidly from proliferating cells. The data presented suggest involvement of HB‐GAM in cell differentiation phenomena rather than in cell proliferation.