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Interaction between oleic acid‐containing pH‐sensitive and plain liposomes Fluorescent spectroscopy studies
Author(s) -
Torchilin V.P.,
Lukyanov A.N.,
Klibanov A.L.,
Omelyanenko V.G.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80663-2
Subject(s) - liposome , oleic acid , chemistry , phosphatidyl choline , ethanolamine , chromatography , fluorescence , phospholipid , biophysics , biochemistry , membrane , biology , physics , quantum mechanics
The energy transfer method has been applied to study the interaction between pH‐sensitive liposomes (phosphatidyl ethanolamine/oleic acid/cholesterol, 4:2:4 molar ratio) and plain liposomes (phosphatidyl choline/phosphatidyl ethanolamine/cholesterol, 4:2:3 molar ratio). It was shown that a slow fusion process occurs between two types of liposomes. Also, the transfer of oleic acid from pH‐sensitive liposomes to plain liposomes takes place. This transfer results in the increased permeability of both pH‐sensitive and plain liposomes, facilitating the release of liposome‐entrapped fluorescent dye. The data obtained were used for a possible explanation of the mechanism of intracytoplasmic drug delivery by pH‐sensitive oleic acid‐containing liposomes.