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Intranuclear topological distribution of HIV‐1 trans‐activators
Author(s) -
Miyazaki Yukio,
Takamatsu Tetsurou,
Nosaka Tetsuya,
Fujita Setsuya,
Hatanaka Masakazu
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80642-t
Subject(s) - distribution (mathematics) , human immunodeficiency virus (hiv) , chemistry , physics , topology (electrical circuits) , biology , virology , mathematics , combinatorics , mathematical analysis
Subcellular localization of human immunodeficiency virus type 1 (HIV‐1) Tat and Rev was examined using a confocal laser scanning microscope (CLSM). In transfected COS‐7 cells, Tat resided exclusively in the perinocleolar region, while Rev infiltrated fully into the nucleoli. The chimeric Tat in which the nucleolar targeting signal was replaced by that of Rev, which retains trans ‐acting activity of Tat, remained still in the perinucleolar region as wild‐type Tat. Perinucleolar distribution of Tat protein suggests the existence of a novel nucleolar architecture that affects transcription.