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ATP and GTP as alternative energy sources for vinblastine transport by P‐170 in KB‐V1 plasma membrane vesicles
Author(s) -
Lelong Isabelle H.,
Padmanabhan Raji,
Lovelace Elizabeth,
Pastan Ira,
Gottesman Michael M.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80632-q
Subject(s) - vinblastine , gtp' , vesicle , nucleotide , chemistry , purine , membrane , biochemistry , guanine , biophysics , biology , enzyme , genetics , chemotherapy , gene
Purified plasma membrane vesicles isolated from multidrug‐resistant human KB‐V1 cells accumulate [ 3 H]vinblastine in an energy‐dependent manner. The accumulation of [ 3 H]vinblastine in the presence of ATP is a saturable process. ATP can be replaced by other purine nucleotide triphosphates, of which GTP is the most efficient.