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Volume‐activated Cl − secretion and transepithelial vinblastine secretion mediated by P‐glycoprotein are not correlated in cultured human T 84 intestinal epithelial layers
Author(s) -
McEwan G.T.A.,
Hunter J.,
Hirst B.H.,
Simmons N.L.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80626-r
Subject(s) - secretion , dids , vinblastine , forskolin , chemistry , glycoprotein , p glycoprotein , endocrinology , medicine , biology , biochemistry , stimulation , chemotherapy , membrane , multiple drug resistance , antibiotics
The relationship between the P‐glycoprotein‐mediated vinblastine secretion and cell‐swelling activated Cl − secretion (conductance) in intact epithelial layers of human colonic adenocarcinoma T 84 cells has been investigated. Whereas vinblastine secretion is effectively inhibited by 100 μM 1,9‐dideoxy‐forskolin, volume‐stimulated Cl − secretion is unaffected. In contrast, 100 μM 4,4′‐diisothiocyanostilbene‐2,2′disulphonic acid (DIDS) inhibited the volume‐stimulated Cl − secretion, but was without effect upon transepithelial vinblastine secretion. In addition, it was noted that some epithelial layers failed to express a volume‐stimulated Cl − secretion but maintained a normal level of secretory vinblastine flux.