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Cloning of a human choline kinase cDNA by complementation of the yeast cki mutation
Author(s) -
Hosaka Kohei,
Tanaka Susumu,
Nikawa Jun-ichi,
Yamashita Satoshi
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80625-q
Subject(s) - complementation , complementary dna , yeast , microbiology and biotechnology , biochemistry , cdna library , enzyme , biology , amino acid , peptide sequence , protein fragment complementation assay , molecular cloning , cloning (programming) , choline kinase , gene , mutant , computer science , programming language , phospholipid , phosphatidylcholine , membrane
A human choline kinase cDNA was cloned by complementation of the yeast choline kinase mutation, cki , from a human glioblastoma cDNA expression library. The deduced sequence of the human enzyme comprised 456 amino acids with a calculated relative molecular mass of 52.065. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy‐terminal region, but not much in the amino‐terminal region.