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Extracellular 2‐chloroadenosine and ATP stimulate volume‐sensitive Cl − current and calcium mobilization in human tracheal 9HTEo− cells
Author(s) -
Galietta Luis J.V.,
Rasola Andrea,
Rugolo Michela,
Zottini Michela,
Mastrocola Teresa,
Gruenert Dieter C.,
Romeo Giovanni
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80589-9
Subject(s) - extracellular , extracellular fluid , adenosine , biophysics , chemistry , intracellular , calcium , adenosine receptor , microbiology and biotechnology , purinergic receptor , receptor , biochemistry , biology , agonist , organic chemistry
The perforated‐patch whole‐cell technique was used to record membrane currents in epithelial cells (9HTEo−) obtained from the human tracheal epithelium. Extracellular application of 2‐chloroadenosine and ATP (0.01–100 μM) caused activation of Cl − currents similar to those regulated by cell volume in airway and intestinal cells. This response was inhibited by increasing extracellular osmolality, by omission of extracellular Ca 2+ or by the addition of the A 2 adenosine receptor antagonist 3,7‐dimethyl‐1‐propargylxanthine (DMPX). Fluorimetric measurements with fura‐2 reveal that 2‐chloroadenosine and ATP elicited both a Ca 2+ influx through the plasma membrane and a release from intracellular stores.