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HSP70‐related 65 kDa protein of beet yellows closterovirus is a microtubule‐binding protein
Author(s) -
Karasev Alexander V.,
Kashina Anna S.,
Gelfand Vladimir I.,
Dolja Valerian V.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80578-5
Subject(s) - closterovirus , microtubule , biology , microbiology and biotechnology , hsp70 , biochemistry , gene , heat shock protein , genome
Beet yellows virus (BYV) genome encodes a 65 kDa protein homologous to the HSP70 family of cellular heat‐shock proteins (Agranovsky, A.A., Boyko, V.P., Karasev, A.V., Koonin, E.V. and Dolja, V.V. (1991) J. Mol. Biol. 217, 603–610). The respective gene was cloned and expressed in vitro yielding a product of the expected size (p65). This product was found to bind to the purified microtubules with a binding constant of 4 × 10 −7 M. The binding of p65 was stimulated if ATP presented in the translation mixture was hydrolyzed by apyrase. Removal of the short C‐terminal domains of α‐ and β‐tubulin by subtilisin digestion abolished the binding, demonstrating its specificity. The possible role of p65 association with microtubules in the movement of virus within and/or between plant cells is proposed.